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1. Bioavailability Of Gentamicin In Male Buffalo Calves

by Hasan Raza, S | Dr. Muhammad Sarwar Khan | Dr. Muhammad Ashraf | Prof. Dr. Saghir | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1994Dissertation note: The bioavailability and pharmacokinetics of Gentamicin sulphate were investigated in the same 20-male buffalo calves heal thy after intravenous administration and after intramuscular administration. The blood samples were collected at various time intervals following administration of single dose of 4 mg/kg. The concentrations of Gentamicin in serum samples were determined according to the microbiological assay described by Arret (1971). The plasma concentrations of gentarnicin at different time intervals after injections were plotted on semi-logarithmic graph paper. The bioavailability and pharmacokinetics parameters were calculated according to the method described by Gibaldi (1984). Results were expressed as mean ± SD. The peak concentration of 11.273 ± 0.4976 µg/ml reached in 31.092 ± 1.217 minutes after intramuscular injection. Keeping intravenous as standard the relative bioavailability after injection was 82.5%. The half-life was 97.29 ± 5.259 minutes after I/v injection mean ± SD, volume of distribution was 202.36 ± 8.486 ml/kg/ The volume of distribution at steady stage (Vdss ) was 214.67 ± 20.99 ml/mm. The total body clearance of gentamicin was 1.7382 ± 0.0738 ml/kg. Pharmacokinetics parameters of gentamicin were seemed to be independent of rout of administration at the dosage level applied. The pharmacokinetics evaluation by compartmental method and non-compartmental method was not found significantly different. Availability: Items available for loan: UVAS Library [Call number: 0390,T] (1).

2. Effect Of Different Antimicrobials At Therapeutic Dose Level On Blood Parameters Of Clinically Normal And Diseased Sheep

by Sheraz Bashir | Dr . Muhammad Athan Khan | Dr . Muhammad Ashraf | Dr . Saghir | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2000Dissertation note: The effect of amoxycillin, oxytetracycline and chloramphenicol at therapeutic dose level on the blood parameters of clinically normal and diseased (pyretic) sheep was studied in 18 febrile and 18 non febrile sheep for a period of 3 consecutive days. Amoxycillin treated diseased animals showed a gradual decline in temperature, pulse, respiration rate, neutrophil count, lymphocyte count, monocyte count and total serum protein, while total erythrocyte count, packed cell volume, total leukocyte count, eosinophil count and basophil counts were increased. In oxytetracycline treated affected animals, temperature, pulse, total erythrocyte count, neutrophil count, monocyte count, basophil count and total serum protein decreased while respiration rate, packed cell volume, total leukocyte count, lymphocyte count and eosinophil count increased gradually with time. In chloramphenicol treated pyretic animals temperature, pulse, respiration rate, total erythrocyte count, total leukocyte count, neutrophil count, lymphocyte count, basophil count and total serum protein decreased and packed cell volume and eosinophils increased while no effect was noted on monocyte count from day zero progressively. In amoxycillin treated non affected animals, total erythrocyte count, packed cell volume, neutrophils, lymphocytes, monocytes and eosinophil increased while pulse, total leukocyte count and total serum protein decreased and no effect was observed on temperature, respiration and basophil count. In oxytetracycline treated normal animals pulse, respiration rate, total erythrocyte count, packed cell volume, total leukocyte count and eosinophil increased while neutrophil and total serum protein decreased gradually and there was no effect on temperature, lymphocytes, monocytes and basophils. In chloramphenicol treated clinically normal animals, pulse, lymphocytes, monocytes and eosinophH increased while total erythrocyte count, total leukocyte count, neutrophil and total serum protein values decreased from day zero to onward. But there was no effect on temperature, respiration rate, packed cell volume and basophil count from day zero to day second. Availability: Items available for loan: UVAS Library [Call number: 0686,T] (1).



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